《植物生理学报》 2011, 47(1): 80-84

牡丹品种‘胡红’开花和衰老过程中花瓣脂肪酶活性的变化

史国安^1,*, 郭香凤¹, 高双成¹, 李昕¹, 李友军¹, 包满珠²

1 河南科技大学农学院, 洛阳市牡丹生物学重点实验室, 河南洛阳 471003; 2 华中农业大学园艺林学学院, 园艺植物生物学教 育部重点实验室, 武汉 430070

通信作者：史国安；E-mail: gashi1963@163.com；Tel: 0379-64283552

摘　要：

以牡丹品种‘胡红’为材料, 采用改良铜皂法测定了牡丹花发育过程中花瓣脂肪酶活性的变化。结果表明, 牡丹花瓣粗 脂肪酶作用的最适 pH 为 6.5, 最适温度为 35 ℃。OP 乳化剂提高酶活性 3.5 倍, Mg2+ 对酶活性没有影响。露色期(I 级)脂肪酶 活性和游离脂肪酸含量最高, 之后各时期酶活性逐渐下降并维持在低水平; 而游离脂肪酸含量在盛开期(V级)有一小高峰

关键词：牡丹; 开花和衰老; 花瓣; 脂肪酶活性

收稿：2010-10-11   修定：2010-11-28

资助：国家自然科学基金项目(30740013)、河南省自然科学基金项目(0611030600)、河南省重大科技专项(091100110100)和洛阳市科技支撑计划项目(0901063A)。

Change in Lipase Activity in Petal during Flowering and Flower Senescence of Paeonia suffruticosa Andr. cv. ‘Huhong’

SHI Guo-An^1,*, GUO Xiang-Feng¹, GAO Shuang-Cheng¹, LI Xin¹, LI ou-Jun¹, BAO Man-Zhu²

¹College of Agriculture, Luoyang Key Laboratory of Peony Biology, Henan University of Science and Technology, Luoyang 471003, China; ²Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticultural and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: SHI Guo-An; E-mail: gashi1963@163.com; Tel: 0379-64283552

Abstract:

Using peony (Paeonia suffruticosa) cv. ‘Huhong’ as the experimental material, we investigated the change in lipase activity of petal during flowering and flower senescence by the improved copper soap method. The results indicated that the optimum pH value was 6.5 and that optimum temperature was 35 ℃ for lipase activity. OP could increase the lipase activity by 3.5- fold, but Mg²⁺ had no effect. The peaks of lipase activity and free fatty acid content occurred at bud color-occurring stage. The lipase activity kept at low level from bud soft stage to wilting, peak of free fatty acid content occurred at full opening stage.

Key words: Paeonia suffruticosa; flowering and flower senescence; petal; lipase activity